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The Regulation of DEK in Human Endometrial Decidualization
Wu Mengyun, Wang Yingxiong, Liu Xueqing*
Laboratory of Reproductive Biology, School of Public Health and Management Chongqing Medical University, Chongqing 400016, China
Abstract: In this study， to investigate the regulation of endometrial decidualization by Drosophila Eph kinase (DEK) a DNA-binding protein， the expression pattern of DEK in human tissue (proliferative， secretory and decidualization) was detected by Real-time quantitative polymerase chain reaction (qPCR)， immunohistochemistry (IHC) and Western blot. Human uterine stromal and decidual cells were inhibited the expression of DEK. Then the proliferation， differentiation， apoptosis and DNA damage of cells were detected. Our results revealed that the expression pattern of DEK mRNA in decidualization was significantly lower than that in proliferative and secretory groups. The expression pattern of DEK protein in decidualization was significantly higher than that in proliferative and secretory groups. DEK inhibited in stromal cells causes decrease in proliferation and differentiation ability.DEK inhibited in decidual cells causes increases apoptosis and DNA damage. Taken together， in the present study，we primarily indicated that DEK possibly through regulation of cell decidualization to participate in embryo implantation process. DEK through the regulation of cell proliferation， differentiation， apoptosis and DNA damage is involved in the regulation of decidualization.
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