The Biological Effects of Sec23a Knockdown in Human Oligometastatic Breast Cancer Cells In Vitro

Abstract: The MDA-435-OL-Sec23a-GFP cell line in which the Sec23a was knocked down and the MDA-435-OL-LV3NC-GFP cell line as the negative control were derived from the oligometastatic cell lines MDA-435-OL obtained by the breast cancer cell line MDA-MB-435 metastasizes to the mouse lungs through the lentivirus containing recombinant plasmids. Thereafter， the effects of Sec23a stable knockdown on cell proliferation， migration， invasion and clonogenicity were measured in vitro by the Cell Counting Kit-8 (CCK-8) assay， Transwell migration assay， matrigel invasion assay and agar clone formation assay， respectively. The results showed that the numbers of migrated cells (58.50±2.81 vs 39.60±3.21)， invaded cells (54.40±3.33 vs 34.60±1.44) and formed cell clones (0.67±0.05 vs 0.37±0.03) increased significantly(P<0.001) after stable Sec23a knockdown. In contrast， cell proliferation rate was not significantly affected (28.23 h vs 28.32 h， P>0.05). The abilities of cell migration， cell invasion and cell clone formation were enhanced while the cell growth was not altered after Sec23a stable knockdown in the oligometastatic breast cancer cells. Taken together， these results indicate that the stable knockdown of Sec23a enhances cancer biological features of human oligometastatic breast cancer cells.

CSTR: 32200.14.cjcb.2016.12.0002