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Overexpression of RORα Influence Target Genes of Wnt/β-catenin Signaling Pathway in Human Gastric MGC803 Cells
Su Jian1,2#, Zhao Xiaohong2#, Liu Fang2, Xia Hong2, Su Bo2, Zeng Xi2, Ling Hui2, Su Qi2*
1Department of Pathology, the Second Affiliated Hospital, University of South China, Hengyang 421001, China;
2Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, University of South China, Hengyang 421001, China
2Cancer Research Institute, Center for Gastric Cancer Research of Hunan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, University of South China, Hengyang 421001, China
Abstract: This work was aimed to investigate the effect of target genes of Wnt/β-catenin signaling pathway in human gastric MGC803 cells by overexpression of RORα. The proliferation of MGC803 cells was detected by MTT. The expressions of signal molecules in Wnt/β-catenin signaling pathway were tested by RT-PCR， Western blot and co-immunoprecipitation. Luciferase report gene assay was used to assess the promoter activity of c-Myc. MTT showed that the proliferation of MGC803 cells with overexpression of RORα was significantly attenuated compared with control group (P<0.05). The mRNA and protein levels of Wnt1 were respectively downregulation (P<0.05). And， the β-catenin mRNA and protein level were not significant deviation (P>0.05). Co-immunoprecipitation exhibitet that RORα bind to β-catenin was significant increased in MGC803 cells with overexpression of RORα (P<0.05).However， the expression of intranuclear β-catenin was markedly downregulated (P<0.05). At the meantime， the expression of TCF-4 (T cell factor-4) was downregulated in MGC803 cells with overexpression of RORα (P<0.05).MGC803 cells with overexpression of RORα has the decrease of mRNA and proteins levels of target genes by Wnt/β-catenin signaling pathway， such as Axin， c-Myc and c-Jun， and inhibit the promoter activity of c-Myc. These results indicated that overexpression of RORα in MGC803 cells could suppress the expression of target genes by Wnt/β-catenin signaling pathway， and then inhibit the proliferation in MGC803 cells.
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