Home > Browse Issues > Vol.38 No.11
Cell Interaction Influences Maturation of Induced Pluripotent Stem Cell Derived Hepatocyte
Wang Ding1,2*, Guo Liyuan1, Li Weiqiang2
1Key Laboratory for Major Obstetric Diseases of Guangdong Province, Experiment Department of Gynaecology and Obsetrics Intitute, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China;
2Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China
2Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China
Abstract: Study of pluripotent stem cell hepatocyte committed differentiation would be helpful to understand the mechanism of liver regeneration and obtain the biomaterials for regeneration medicine and drug screening. We established four stage inductions and compared the cell morphology and hallmarks of each differentiation stage between passage and no-passage. By committed differentiation， we derived ALB (albumin) positive mature hepatocyte from iPSc (induced pluripotent stem cell). The phenotype of cells with no-passage was closer to mature hepatic cells. The two groups had AFP (alpha-fetoprotein) and ALB positive cells. There was no significant difference between the expression of GATA6 and HNF4A， but expression of AFP and ALB were significant higher in no-passage group than passage group. It concluded that pluripotent stem cell hepatocyte committed differentiation was one comprehensive process， which involving cytokine single， cell-to-cell and cell-tomatrix communication， keep the established cell interaction would benefit the hepatocyte mature.
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