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Study in the Isolation， Identification and Biological Characteristics of Adipose-Derived Stem Cells in Rat
Yue Yongli, Zhang Xiang, Zhang Lichun, Yu Haiquan*
Key Laboratory of Ministry of Education of China for Mammal Reproductive Biology and Biotechnology,Inner Mongolia University, Hohhot 010021, China
Abstract: As a class of adult stem cells， adipose-derived mesenchymal stem cells (ADSCs) have multipotent differentiation potential and promising prospect in tissue repair. To maintain the proliferation and differentiation potential in vitro is important for the medical application of ADSCs. ADSCs were isolated from rat inguinal fat pads and assessed the biological feature including growth curve， population doubling time and colony formation assay. The optimal medium formulation was confirmed on the ADSCs behavior by different fetal bovine serum (FBS) with different concentration.The rat ADSCs were also detected by immunofluorescent staining， flow cytometry and induction by specific medium.The results showed that the most rapid proliferation and population doubling time occured in 20% FBS in which the rate of the cell colony formation was (27.33±0.58)% of rat ADSCs. The ratios of CD44， CD90 and CD106 positive cells in rat ADSCs were 99.12%， 99.59% and 65.81%， respectively， while CD34 was negative. The results of histochemical staining of Oil Red O， alkaline phosphatase and Alcian Blue staining demonstrated that rat ADSCs were capable to differentiate towards adipocyte， bone and cartilage. The results above showed that rat ADSCs were established successfully.
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