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The Application of Callus Induction and Agrobacterium-Mediated Transformation Technology of Brachypodium distachyon in the Undergraduate Experimental Course
Liu Yiran, Sun Xiaofei, Men Shuzhen*
Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Abstract: The technology of embryogenic callus induction and regeneration plays an important role in plant breeding and selection of improved variety. However， undergraduate experimental courses lack the relevant content. In this project， we choose model monocot plant Brachypodium distachyon as experimental material， and integrated phenotype observation of Brachypodium distachyon， callus induction and redifferentiation， and Agrobacterium-mediated transformation into one project. We did practice in detail， and then designed a series of suitable experimental procedures for undergraduate courses. This experiment included technologies of callus induction and genetic transformation， and was designed to ensure attending students to get positive results， improving their experimental skills， and broaden their knowledge. We furthermore provided valuable suggestions for the popularization of this project in undergraduate experimental course.
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