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miR-486 Regulated Lipid Metabolism through Targeting Pten/Foxo1 in Mouse Hepatic Cell Line
Tian Haiying1#, Mo Suo1#, Liu Qiuju1, Shi Mengru1, Jin Jing1, Lü Jianxin1,2*, Li Wei1,2*
1Wenzhou Medical University School of Laboratory Medicine and Life Science, Wenzhou 325035, China;
2Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou 325035, China
2Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou 325035, China
Abstract: In order to investigate the regulatory effects and mechanism of miR-486 on triglyceride (TG) and very low density lipoprotein (VLDL) synthesis in mouse liver cells， the miR-486 mimic or antago were infected into Hepa1-6 mouse hepatoma cells through adenovirus vector， then Pten (phosphatase and tensin homolog)/Foxo1 (forkhead box O1) and lipid metabolism were measured. The result showed that overexpression of miR-486 (P<0.001) attenuated the expression of PTEN/FoxO1 and the VLDL content (P<0.01)， and increased the TG content (P<0.05). Conversely， down-regulation of miR-486， increased the expression of PTEN/FoxO1 and the VLDL content (P<0.01)， and reduced the TG content (P<0.05). It was concluded that miR-486 can affect lipid metabolism in mouse liver cells by regulating its potential targets Pten/Foxo1.
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