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Cloning and Expression of Linalool Synthase Gene in Freesia
Fan Ronghui1,2,3, Huang Minling1,2,3*, Zhong Huaiqin1,2,3, Luo Yuanhua1,2,3
1Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou 350013, China;
2Flowers Research Center, Fujian Academy of Agricultural Science, Fuzhou 350013, China;
3Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou 350013, China
2Flowers Research Center, Fujian Academy of Agricultural Science, Fuzhou 350013, China;
3Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou 350013, China
Abstract: Linalool is the main components of the floral scent emitted from Freesia. In order to explore the synthesis and metabolic mechanism of linalool synthase (LIS)， the open reading frame (ORF) sequence of LIS gene was cloned from petals of Freesia ‘Jintong’ using RT-PCR. The cDNA included a whole ORF of 1 779 bp. The amino acid was highly conserved compared with other LIS homologues and shared up to 40%~95% homology with other LIS. Phylogenetic analysis indicated that LIS in ‘Jintong’ was clustered together with LIS of Freesia hybrid firstly and was more related to monoterpene synthases of Lilium sp.， Hedychium coronarium and Musa acuminata.The result from fluorescent quantitative PCR analysis indicated that LIS was highly expressed in early flowering period， and the transcriptional level was highest in White Wing-2 and petals.
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