Home > Browse Issues > Vol.38 No.9
Application of Extracellular Flux Analyzer in the Studies of Cancer Cell Metabolism
Wang Chunlong1#, Guo Miaomiao2#, Wu Yiqi2#, Chen Fuhong2, Lan Linhua2, Xu Cuicui2, Lou Kangcheng3,Shen Kai2, Xu Hao2, Yu Xiaomin2, Liu Yongzhang2, Lü Bin2*, Xie Hongli1*
1The First Clinical Medical College, Wenzhou Medical University, Wenzhou 325035, China;
2School of Laboratory Medicine and Life Sciences, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou 325035, China;
3The Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325035, China
2School of Laboratory Medicine and Life Sciences, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou 325035, China;
3The Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325035, China
Abstract: In recent years， growing studies are focusing on cancer therapy via targeting key molecules in cancer cell metabolism. The traditional methods for the study of oxidative phosphorylation and aerobic glycolysis of cancer cells are oxygen consumption rate (OCR) measurement by Clark oxygen electrode method and the determination of crucial inter-mediate substrates， such as L-lactate and glucose. However， the traditional methods used to determine the variation of cell bioenergetics are very complicated and time consuming. Here， we introduced a novel method which works effectively in the study of cellular bioenergetics and investigated the cellular bioenergetics of cancer cells with or without doxorubicin and certain drugs pretreatment to demonstrate the advantage of Extracellular Flux Analyzer. Our data indicated that the concentration of carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) and the cell density are crucial for the oxygen consumption detection. Additionally， our results showed that doxorubicin inhibits tumor cell growth may also through disrupting aerobic glycolysis and mitochondrial respiration.Overall， our work may provide reference for the investigation of cellular bioenergetics.
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