Home > Browse Issues > Vol.38 No.9
Bovine Mammary Epithelial Cells Isolated and Cultured by Collagenase I Digestion Method In Vitro
Xu Dandan, Sun Zhipeng, Zhang Xu, Liu Dongyu, Xu Xiaonan, Wang Le, Chen Jia, Wang Jianfa, Yang Bin, Wu Rui*
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract: The aim of this study is to isolate and culture bovine mammary epithelial cells. We used the method of collagenase I digestion to culture bovine mammary epithelial cells without adding exogenous hormones and growth factors. Bovine mammary epithelial cells were purified by different time digestion method and different speed adherence method. The level of β-casein (CNS2) in cells were detected by Western blot. Cells were identified by immunofluorescence. The results showed that the cells had typical cobblestone-like morphology， and positively expressed cytokeratin-18 (CK-18)， negative expressed vimentin. The cells of 15th passage were recovered， and still grown in good condition. This result indicated that collagenase I digestion method can be used to obtain bovine mammary epithelial cells without adding exogenous hormones and growth factors.
CN
EN