Home > Browse Issues > Vol.38 No.8
Establishment and Characterization of Inducible miR-302s Knock-in Mice
Yang Hua1, Ni liangrong3, Wan Yinghan3, Zhou Yunhe1,2, Fei Jian1,3*
1School of Life Science and Technology, Tongji University, Shanghai 200092, China;
2Department of Physical Education,Tongji University, Shanghai 200092, China;
3Shanghai Research Center for Model Organisms, Shanghai 201203, China
2Department of Physical Education,Tongji University, Shanghai 200092, China;
3Shanghai Research Center for Model Organisms, Shanghai 201203, China
Abstract: Induced pluripotent stem cell (iPSC) is an important method of stem-cell therapy. Compared with traditional Oct4/Sox2/Klf4/Myc-mediated method， miR-302s-induced iPSC displays higher inductive efficiency and lower tumorigenicity， suggesting that it may have broad prospect for clinical application. In order to evaluate the biological roles of miR-302s overexpression in vivo， and provide experimental support for further clinical transformation， we constructed drug-inducible miR-302s knock-in mice. The results showed that the knock-in miR-302s cluster could be drug-regulated and expressed stably in vivo. Preliminary phenotypic analysis revealed that overexpression of miR-302s may lead to lipoprotein or purine metabolism disorder. This mice model could be a useful tool for investigate the function of miR-302s and explore its feasibility into the clinic.
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