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Silent Information Regulator 3 Promotes Apoptosis of Hepatocellular Carcinoma Cells and Its Mechanism
Tao Ying1, Chen Juan2*
1The People’s Liberation Army 161 Hospital, Wuhan 430012, China;
2Key Laboratory of Molecular Biology on Infection Diseases of Ministry of Education, Chongqing Medical University, Chongqing 400016, China
2Key Laboratory of Molecular Biology on Infection Diseases of Ministry of Education, Chongqing Medical University, Chongqing 400016, China
Abstract: This study investigated the effect of silent information regulator 3 (sirtuin 3， SIRT3) on the apoptosis of hepatocellular carcinoma (HCC) cells， and the molecular mechanism of SIRT3 regulating HCC cells apoptosis. The influence of SIRT3 overexpression on the apoptosis of HCC cells (SMMC-7721 and SK-Hep-1) was measured by flow cytometry (FCM). SIRT3 expression was downregulated by siRNA targeting SIRT3， then the apoptosis induced by siSIRT3 was detected by FCM. The effect of SIRT3 on the mRNA level of Bcl-2 family members was assayed by Real-time PCR. The protein level of targeting Bcl-2 family member was further tested by Western blot after SIRT3 overexpressed and knockdown. The role of Bcl-2 family members in the HCC cells apoptosis induced by SIRT3 was analyzed by FCM. Our results showed that SIRT3 overexpression promoted HCC cells apoptosis. SIRT3 silent inhibited HCC cells apoptosis. SIRT3 increased the mRNA and protein level of Bax.SIRT3 knockdown inhibited the expression of Bax protein. Bax knockdown significantly decreased the apoptosis ration in cells overexpressed SIRT3. Together， these data indicated that SIRT3 induced HCC cells apoptosis through apoptosis-regulatory gene Bax.
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