Microarray Analysis of Differentially Expressed Genes Related to Abiotic Stress in SlNAC10-Transgenic Arabidopsis

Abstract: NACs (NAM、ATAF1/2 and CUC2) were plant specific transcription factors and played essential roles in plant morphogenesis， growth and development， and abiotic stress responses. In this study， microarray was used to analyze differentially expressed genes related abiotic stress in the wild-type (WT) and SlNAC10-transgenic Arabidopsis (L1)， and the expression patterns of selected differentially expressed genes were further identified by quantitative Real-time PCR. A total of 4 054 differentially expressed genes were found， 15 of them were related to abiotic stress， and 14 were abiotic stress-related TFs. These genes were involved in responding to osmotic stress，high salinity， cold， heat， high light intensity respectively. The differentially expressed genes were subjected to GO (Gene Ontology) analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways enrichment analysis.These genes enriched in 13 annotations related to abiotic stress and involved in 96 metabolic pathways， including plant hormone signal transduction， arginine and proline metabolism， indole alkaloid biosynthesis and glutathione metabolism. These results indicated that SlNAC10 regulated the expression of downstream genes directly or indirectly and enhanced plant resistance to abiotic stress

CSTR: 32200.14.cjcb.2016.08.0007