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Effects of Hypoxia on the Growth， Senescence and Apoptosis of Human Cartilage Endplate Derived Stem Cells In Vitro
Cheng Nan, Liu Huan, Yao Yuan, Li Jie, Zhou Yue*
Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Abstract: In this study， we obtained human cartilage endplate derived stem cells (CESCs) via primary culture. The effects of hypoxia (1% O2) on growth， senescence， apoptosis and cell cycle of CESCs were investigated by WST-1 cell proliferation and cytotoxicity assay kit， Edu cell proliferation assay kit， β-galactosidase staining， flow cytometry， respectively. The results showed that proliferation of CESCs was significantly promoted， senescence and apoptosis of CESCs were significantly inhibited cultured hypoxia for 72 h， compared with the normoxia (21%O2) group. Hypoxia culture affected the cell cycle of CESCs， cell proportion of G1 phase was firstly increased then decreased， and the cell proportion of S and G2/M phases were firstly decreased then increased. The results demonstrated that hypoxia could promote CESCs proliferation and inhibit CESCs senescence and apoptosis.
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