Deubiquitinating Enzyme nonstop Regulates Cone Cells Development in Drosophila Eye

Abstract: Deubiquitinating enzymes (DUBs) are kinds of protein involved in the regulation of protein stability in eukaryotic cells， and play important roles in cell processes including the regulation of cell cycle， cell signal transduction， protein degradation， DNA repair and gene expression. The function of DUBs is to cleave ubiquitin (Ub) from Ub-conjugated protein substrates， and dysfunction of DUBs causes many diseases. In the current study， we used Drosophila as a model to explore the function of deubiquitinating enzyme nonstop (not) in cone cells development. Our results showed that the protein level of Cut was reduced in not mutant cells in eye imaginal disc. In the pupal eye， knockout not affected the differentiation of cone cells and primary pigment cells. The further study showed that the expression level of D-Pax2 was changed and the differentiation of R1/R6 photoreceptor was abnormal in not mutant cells. However， the protein level of Lozenge (Lz) and the avtivity of epidermal growth factor receptor (EGFR) and Notch signaling were not affected in not mutant cells. Taken together， our result indicated that not may affect the differentiation of cone cells and primary pigment cells by regulating the expression level of D-Pax2 and the differentiation of R1/R6 photoreceptor.

CSTR: 32200.14.cjcb.2016.08.0002