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Effects of Fumonisin on TAP-1 Expression of Human Peripheral Blood Mononuclear Cells in Vitro
Ling-Xiao Xing, Xiang-Hong Zhang*, Yue-Hong Li, Lian-Fu Zuo1, Xia Yan, Jun-Ling Wang, Feng-Rong Wang
Laboratory of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China; 1Laboratory of Cytology, Institute of Hebei Province Oncology, Shijiazhuang 050011, China
Abstract: To explore the effects of fumonisin (FB1) on TAP-1 (transporter associated with antigen processing) expression of human peripheral blood mononuclear cells (hPBMC) in vitro. The expression of TAP-1 of hPBMC by FB1 pretreatment at three different dosages (0， 10 and 50 μmol/L) were detected with flow cytometry (FCM)， Western blot and semi-RT-PCR respectively. TAP-1 mRNA was decreased in 10 and 50 μmol/L FB1 treated groups than that in control group. FCM quantitative analysis showed that at protein level， TAP-1 expression (as expressed by FI) of hPBMC at FB1 treated groups were lower than that of control， especially in 50 μmol/L FB1 treated groups (P<0.05). Western blot results further confirmed the above results. 10 and 50 μmol/L FB1 treatment could inhibit the expression of TAP-1 at mRNA and protein level.
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