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Establishment of Pancreatic Cancer Cell Line Stably Expressing Luciferase Mediated by Nucleofector System and Detection with Bioluminescence Imaging System
Tu Jue, Huang Yu, Ling Yun, Qi Yuehan, Chen Minli*
Laboratory Animal Research Center/Comparative Medical Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China
Abstract: The paper aimed to establish a pancreatic cancer cell line which could stably produce luciferase and suit for constructing xenotransplanted tumor model in nude mice. PANC-1 cells were transfected with plasmid (GV258) with luc gene mediated by Lonza NucleofectorTM System and monoclonal cell line PANC-1-LUC was screened with puromycin. The luciferase expression of PANC-1-LUC cells was detected by bioluminescence imaging system in vitro and in vivo. The results showed that the bioluminescence density of PANC-1-LUC cells correlated significantly to the cell numbers in vitro and in vivo (P<0.01). Furthermore， the PANC-1-LUC cells were inoculated subcutaneously into back of nude mice. A week later， the tumor growth and bioluminescence of xenotransplanted tumor were investigated. The high tumorigenecity above 80% was observed and the curve of tumor growth was accorded with Gompertzian curve. The results of weekly bioluminescence detection inferred a significant correlation between total flux value of bioluminescence and volume of xenotransplanted tumor (P<0.05).Overall， we have successfully established a pancreatic cancer cell line， PANC-1-LUC， which could produce luciferase stably and persistently.
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