Home > Browse Issues > Vol.38 No.6
Activity of Internal Ribosome Entry Site in mRNA 5’ Untranslation Region of Transcription Factor GATA3
Tao Yifen1, Ma Jing2, Zhu Ruiyu2, Duan Zuoying1, Jin Jian2*, Li Huazhong1*
1Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;
2School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
2School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
Abstract: GATA3 is a member of GA-TA-binding protein family and plays an important role in cell proliferation and differentiation， and the abnormal expression of GATA3 in cells is also one cause of many tumor formations.In this paper， the mRNA 5′ untranslated region (UTR) of GATA3 was analyzed， and it was found that the UTR was so long as 557 bp and had a complex secondary structure. When the mRNA 5′ UTR of GATA3 was cloned into the bicistronic luciferase plasmid pRL-FL， it was found that the translation level of FL which was mediated by GATA3 mRNA 5′ UTR was significantly augment under the condition of serum starvation. While the GATA3 mRNA 5′ UTR was inserted into the bicistronic luciferase plasmid ΔpRL-FL and transient transfected into cells， the 5′ UTR of GATA3 mRNA had no promoter activity according to the expression of firefly luciferase， which indicated that GATA3 mRNA 5′ UTR had IRES element. By the truncated analysis of GATA3 mRNA 5′ UTR sequence， it was revealed that the 345-557 bp might inhibit the IRES activity of GATA3 and the 95-344 bp might be the main active center of GATA3 mRNA IRES element. It was also found that this IRES activity was various in different cell lines. These results showed that the 5′ UTR of GATA3 mRNA can participate in the regulation of GATA3 gene expression.
CN
EN