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The Regulation of PKC-ERK Signaling Pathway on Heat-induced Monocytic Cell Apoptosis
The Regulation of PKC-ERK Signaling Pathway on Heat-induced Monocytic Cell Apoptosis
Department of Pathophysiology, the Key Lab of Guangdong Proteome Analysis, the Key Lab for Transcriptomics and Proteomics, Ministry of Education, Southern Medical University, Guangzhou 510515, China; 1Department of Histology and Embryology, Sou
Abstract: By using a combined methods including flow cytometric analysis， Western blotting and kinase assay， the effect of PKC-ERK signaling pathway on heat-induced apoptosis in monocytic cell line Raw264.7 was studied. The results demonstrated that PKC was transiently activated 5 minutes after heating and returned to basal level 2 hours later， while PKC was persistently activated by phorbol-12-myristate-13-acetate (PMA). The persistent activation of PKC can inhibit heat-induced Raw264.7 cell apoptosis， while inhibition of PKC promoted heat-induced Raw264.7 cell apoptosis. Furthermore， kinase analysis showed that heat can suppress the activation of ERK， while PMA can induce ERK activation. The inhibitor of PKC， staurosporine， can attenuate the inhibitory effect of PMA on heat-induced Raw264.7 cell apoptosis. Together， the present results indicate that the PKC-ERK signaling pathway can negatively regulate heat-induced Raw264. 7 cell apoptosis.
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