Home > Browse Issues > Vol.38 No.6
The Infection of Reticuloendotheliosis Virus Changed the Protein Composition and Immunomodulation of Exosome
Zhuang Pingping, Wang Xiaoman, Meng Wei, Li Gen, Cheng Ziqiang, Wang Guihua*
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
Abstract: The indirect immunofluorescence， transmission electron microscopy， Western blot， mass spectrometry analysis， ELISA and flow cytometric were used to investigate the reticuloendotheliosis virus (REV) infected DF-1， the morphology and biomarkers of exosome and the effects of REV on protein composition and immunomodulation of exosome from infected DF-1 cells (Exo-R). The results demonstrated that green fluorescence was observed in the cytoplasm of infected DF-1 cells. Isolated exosome was up to 50-100 nm in diameter， with a cup-shape structure， and expressed marker proteins Hsp70 and Tsg101. Compared with exosome from uninfected cells， 58 kinds of different proteins were detected in Exo-R with up-regulated 46 kinds containing env protein gp90 and gag-pol of REV， and down-regulated 12 kinds. Furthermore， the secretion of IL-10 was increased from splenocytes treated by Exo-R. The number of CD4+T and CD8+T was also significantly decreased. The infection of REV induced the protein composition change of exosome， especially those important proteins carried by REV， which was associated with the immunosuppressive effects induced by exosome.
CN
EN