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Effects of miR-92a on the Functions of Endothelial Progenitor Cells Isolated from Rat Bone Marrow
Su Yunjuan1, Sun Yucong1, Chu Hairong2, Li Hong2*, Cui Xiaodong2, Wang gang3, Cheng Min2
1College of Biological Science and Technology, Weifang Medical University, Weifang 261053, China;
2Medicine Research Center, Weifang Medical University, Weifang 261053, China;
3Cognitive Neuroscience Laboratory, Weifang Medical University, Weifang 261053, China
2Medicine Research Center, Weifang Medical University, Weifang 261053, China;
3Cognitive Neuroscience Laboratory, Weifang Medical University, Weifang 261053, China
Abstract: This article aimed to investigate the effects of miR-92a on the migration， adhesion， proliferation and angiogenesis of endothelial progenitor cells isolated from rat bone marrow. Mononuclear cells were isolated by density gradient centrifugation from rat bone marrow and then cultured with EGM-2MV containing 10% fetal bovine serum. The miR-92a mimics， miR-92a inhibitors and their corresponding negative controls (NC) were transfected into 3-5 generations EPCs， namely late EPCs， by LipofectamineTM 3000， respectively. Modified Boyden chamber， adhesion assay， CCK-8 assay and Matrigel were employed to study EPCs migration， adhesion， proliferation and angiogenesis in vitro. miR-92a mimics significantly inhibited EPCs migration and angiogenesis in vitro (P<0.05)， but had no effect on EPCs adhesion and proliferation. However， miR-92a inhibitor obviously promoted EPCs migration and angiogenesis in vitro (P<0.05). miR-92a inhibits the migration and angiogenesis of EPCs in vitro. The results may provide new clinical strategies toward the prevention and/or treatment of cardiovascular diseases by targeting miR-92a and EPCs.
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