Home > Browse Issues > Vol.38 No.6
IQGAP1 Promotes Cell Proliferation through ERK Pathway in Non-small Cell Lung Cancer
Zhao Mingna, Lou Jiatao*
Department of Laboratory, Shanghai Chest Hospital, ShanghaiJiaotong University, Shanghai 200030, China
Abstract: This study investigated the regulation of IQGAP1 on proliferation and ERK pathway in nonsmall cell lung cancer (NSCLC) cells. A549 cells， with low endogenous IQGAP1 expression， were treated with blank， Ad-GFP and Ad-GFP-IQGAP1， respectively. H1299 cells， with high endogenous IQGAP1 expression，were treated with blank， control siRNA and IQGAP1 siRNA， respectively. Then， it was followed by MTT assay analysis for cell proliferation and Western blot analysis for ERK protein level. In addition， the two cell lines were treated with U0126， an ERK1/2 phosphorylation inhibitor. The results indicated that IQGAP1 over expression promotes cell proliferation and up-regulate the p-ERK1/2 expression in A549 cells， while knock-down IQGAP1 results in the opposite scenario in H1299 cells. Meanwhile， U0126 treatment restrained the promotion of IQGAP1 on cell proliferation. These results suggested that IQGAP1 promotes cell proliferation through ERK pathway in NSCLC.
CN
EN