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Microglias Improved Neurotrophicity of Bone Marrow Mesenchymal Stem Cells after being Stimulated with Injured Neurons
Xiao-Guang Luo*, Chun-Lin Ge, Yan Ren, Jin Zhou, Zhe Wu, Rong Yan, Qiu-Shuang Wang, Chao-Dong Zhang
Department of Neurology, First Affiliated Hospital, China Medical University, Shenyang 110001, China
Abstract: Bone marrow mesenchymal stem cells (BMMSCs) were primarily cultured， BV2 and PC12 were sub-cultured to substitute for microglias and neurons respectively. Co-culture was performed with transwells. Stimulated with injured PC12， BV2 was able to promote the neurotrophicity of BMMSCs， whose supernatant reduced PC12 apoptosis significantly when compared with control [(35.9±13.5)%， (95.1±26.6)% respectively， P<0.05]， and was of the highest basic fibroblast growth factor (bFGF) concentration among groups which was significantly different from that of control [(34.0±10.0) pg/ml， (20.3±7.1) pg/ml respectively， P<0.05]. We demonstrated that when stimulated with injured neurons， microglias improved the neurotrophicity of BMMSCs.
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