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Cardiomyocyte Hypertrophy Induced by Cultured Rat Mesenchymal Stem Cells Conditioned Medium
Rui-Xia Xu, Xi Chen, Jing-Hai Chen, Yu Han, Bian-Mei Han*
Research Center for Cardiovascular Regenerative Medicine, the Ministry of Health, Cardiovascular Institute and Fu-Wai Hospital, Chinese Academy of Medical Sciences, Beijing 100037, China
Abstract: This study examined the hypertrophic effect of mesenchymal stem cells-conditioned medium (MSCs-CM) on neonatal rat cardiomyocytes. Rat MSCs were cultured with serum-free medium under hypoxic condition for 3 h， 6 h， 9 h and 24 h， and then the conditioned medium were collected separately. Cultured neonate rat cardiomyocytes were examined by 3[H]-leucine incorporation， ANF-luciferase expression， F-actin staining and cell area measurement after stimulated with the above MSCs-CM for 48 h. Compared with control group， the total protein content， 3[H]-leucine incorporation， ANF-luciferase expression and cell area were obviously increased in MSCs-CM stimulated groups. These results demonstrated that MSCs-CM induced cardiomyocyte hypertrophy in a paracrine manner and implied that this effect likely involved in the improvement of heart function after MSCs transplantation.
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