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Effects of Fasting and Re-feeding on the Expression of I-FABPb in Larimichthys crocea
Huang Hongli1, Xue Liangyi1,2*, Wang Jun3
1College of Marine Sciences, Ningbo University, Ningbo 315211, China; 2Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315211, China; 3College of Science and Technology, Ningbo University, Ningbo 315212, China
Abstract: To study the role and its molecular mechanism of intestines fatty acid-binding protein gene (IFABP) in teleost lipid metabolism， 1 362 bp I-FABP gene was cloned from large yellow croaker intestines tissue.I-FABPb expression was detected in muscle， liver， intestine， stomach， gonad， gill， brain and heart tissues by Realtime fluorescent quantitative PCR (qRT-PCR). The effects of fasting and re-feeding on the expression of I-FABPb in large yellow croaker intestines， muscle and liver tissues were also analyzed. Results showed that the large yellow croaker I-FABPb was widely expressed in all tested eight tissues， and most abundant in intestine tissue. Fasting had a significant influence on the expression of I-FABPb in large yellow croaker intestine， muscle and liver tissues， of which the most obvious influence was in intestine tissue. The expression of I-FABPb in all three tissues showed a same tendency， increase at first and then decrease (P<0.05) during fasting. I-FABPb expression level significantly increased after re-feeding treatment (P<0.05) in the three tissues， and also was significantly higher than the level of fasting 0 day (P<0.05). It implied that fasting and re-feeding significantly affected fat metabolism in large yellow croaker， and I-FABPb played an important role in intestinal fat metabolism.
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