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Cloning and Expression Analysis of a Lycopene Beta-cyclase Gene in Strelitzia reginae Banks
Fan Ronghui1,2,3, Huang Minling1,2,3*, Zhong Huaiqin1,2,3, Lin Bing1,2,3
1Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou 350013, China; 2Flowers Research Center,Fujian Academy of Agricultural Science, Fuzhou 350013, China; 3Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou 350013, China
Abstract: In this study， the full-length cDNA sequence of LCYB (lycopene beta-cyclase) gene (SrLCYB) was cloned from yellow sepals of Strelitzia reginae Banks using RACE techniques. The cDNA sequence was 1 908 bp in total length and included a whole open reading frame of 1 515 bp， encoding a polypeptide containing 504 amino acids.Its amino acid sequence was highly conserved compared with other LCYB homologues and shared up to 82% homology with LCYB from Lilium lancifolium. Phylogenetic analysis indicated that SrLCYB was more related to LCYB from Musa spp. AAB Group. The result from fluorescent quantitative PCR analysis indicated that SrLCYB was highly expressed in early flowering period and blooming period， the transcript level was highest in yellow sepals. The result from UPLC analysis indicated that beta-carotene contents were the highest in yellow sepals and in blooming period.These results suggested that the change in SrLCYB in expression levels was positively related to beta-carotene contents.
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