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Effects of Autocrine Motility Factor (AMF) on the Migration and Invasion of
Glioblastoma U251 Cells and Their Mechanism
Li Yang1, Tang Ning2, Liu Zheyu3, Sun Zheng3*
1Neurosurgery Department, The First Affiliated Hospital of Dalian Medical University, Dalian 116001, China; 2College of Pharmacy, Dalian Medical University, Dalian 116044, China; 3Institute of Integrative Medicine, Dalian Medical University, Dalian 116044, China
Abstract: To explore the effects of autocrine motility factor (AMF) on migration and invasion of U251 cells and their related mechanisms， the expression of AMF was interfered by siRNA. The expression of AMF in U251 cells was detected by RT-PCR and Western blot. Wound healing assay and transwell invasion were used to detect the migration and invasion ability of U251 cells. Western blot was used to detect the protein levels of total Akt， p-Akt， Sox2， MMP-2 and MMP-9 before and after down regulation of AMF. Our results showed that the migration and invasion ability of U251 cells， which AMF was down-regulated successfully， has been significantly inhibited. Protein levels of phosphorylation Akt， Sox2， MMP-2 and MMP-9 were decreased significantly compared with the control group. These results indicated that AMF has played an important role in inhibiting the migration and invasion of U251 cells， through down-regulation the expression of PI3K/Akt pathway， Sox2， MMP-2 and MMP-9 proteins.
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