The Effects of Dimethyl Sulfoxide on the Differentiation of Cardiomyocyte from P19 Cells in Vitro

Abstract: P19 cells were induced to differentiate toward cardiomyocytes in vitro by the different concentrations of dimethyl sulfoxide (DMSO). Immunocyochemistry， flow cytometry and RT-PCR test were used to detect the expression of α-sarcomeric actin (α-SA)、cardiac troponin T (cTnT) and GATA-4， α-myosin heavy chain (α-MHC) mRNA in differentiated cells. It was shown that DMSO treatment together with suspension culture can induce P19 cells to differentiate into spontaneously contracting cardiomyocytes. The cells shown α-SA- and cTnT-positive and expressed cardiomyocyte specific GATA-4 and α-MHC mRNA. The fluorescence intension of α-SA and cTnT positive cells and the expression of GATA-4 and α-MHC mRNA in 1.0% DMSO treated group were significantly higher than that in 0.5% and 0.8% DMSO treated group. Our data suggested that the differentiation efficiency of P19 cells into cardiomyocytes is related with the concentration of DMSO.

CSTR: 32200.14.cjcb.2007.04.0025