Home > Browse Issues > Vol.38 No.2
Application of DNA Flow Cytometry to Plant Genetics and Breeding
Jin Liang1*, Xu Weiwei2, Li Xiaobai3, Liu Jianxin1, Tian Danqing1, Ge Yaying1, Pan Xiaoyun1, Wang Weiyong1
1Research & Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Zhengjiang Xiaoshan Cotton and Flax Research Institute, Hangzhou 311202, China;
2Cixi Agricultural Technology Extension Center, Cixi 315300, China;
3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2Cixi Agricultural Technology Extension Center, Cixi 315300, China;
3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract: DNA flow cytometry is a method for analysis and estimation of DNA content of isolated cell nuclei. The keys to this method include preparation of aqueous suspensions of intact nuclei， staining the nuclei with a nucleic acid fluorochrome， and classification of the stained nuclei based on their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid， DNA flow cytometry has become an important tool for plant ploidy screening， estimation of genome size and determination of reproductive pathway and so on. In this review we gave a brief introduction of the basic principles of DNA flow cytometry， and reviewed its applications in plant genetics and breeding.
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