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Rapid Effects of Estrogen on Intracelluar Calcium of Mouse Dormant Blastocyst
Lin-Lin Yu, Ying Hu, Jin-Hu Zhang, Ya-Ping He, Li-Min Yue*
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China
Abstract: We use Fluo-3/AM， a fluorescent probe of calcium， to sign intracellular calcium; laser scanning confocal microscope， to continuously detect the dynamic intracellular calcium concentration ([Ca2+]i) in blastocyst caused by 17beta-estradiol (17β-E2); and fluorescent microscope， to check out the alterations of [Ca2+]i induced by E2-BSA that conjugated with bovine serum albumin， and also by 17β-E2 with Ca2+-free M2， tamoxifen (a traditional block agent of estrogen receptor)， and U73122 (a specific inhibitor of phospholipase C) respectively. We found that both 17β-E2 and E2-BSA could increase the [Ca2+]i of blastocyst rapidly， while this increasing of [Ca2+]i was independent on the extra calcium in the incubation medium and it can be blocked by specific inhibitor of phospholipase C but not the traditional block agent of estrogen receptor.
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