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Generation of Non-integrated iPSCs and Optimized Its Hematopoietic Differentiation System In Vitro
Fan Di, He Wenyin, Song Bing, Niu Xiaohua, Ou Zhanhui, Chen Yuchang, Sun Xiaofang*
The Third Hospital Affiliated of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou 510150, China
Abstract: Induced pluripotent stem cells (iPSCs) can be differentiated into a various cells， which has broad prospects in the treatment， drug screening and disease study. Differentiated hematopoietic stem cell (HSC) in vitro can provide a new source of HSC for the clinical application and improve the efficiency of hematopoietic differentiation from iPSCs. In this study， we reprogramed normal peripheral blood mononuclear cells (PBMCs) to iPSCs by non viral vector， then differentiated the iPSCs into HSC by co-culutured with OP9 cells. The results showed that the reprogrammed iPSCs could be stably passaged in vitro and differentiated into the three germ layers in vivo. Co-culture with OP9 cells， the iPSCs can be differentiated into hematopoietic stem/progenitor cells， and added cytokines could increase the differentiation efficiency. This results improving the efficiency of differentiation iPSCs to HSC provides important experimental basis for future application clinically.
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