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Long Non-coding RNA PCGEM1 Promotes the Proliferation and Migration of Human Pancreatic Cancer Cells
Xu Min1, Ni Xin1, Ge Lu1, Liu Xin1, Zhou Hailang1, Huang Hongmei1, Jiang Xiaomeng1, Peng Wanxin2, Zhang Youli1, Gong Aihua2*
1Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China;
2Department of Basic Medical Sciences, Jiangsu University, Zhenjiang 212001, China
2Department of Basic Medical Sciences, Jiangsu University, Zhenjiang 212001, China
Abstract: The aim of the present study was to investigate the effect of lncRNA PCGEM1 on proliferation and migration in pancreatic cancer cells in human pancreatic cancer cells. The expression level of PCGEM1 in SW1990， BxPC3 and PaTu8988 cell lines and in human pancreatic cancer tissues and their adjacent non-cancer tissues were detected by qRT-PCR. The pCDH-PCGEM1 was transfected into either BxPC3 or PaTu8988 to increase the expression level of PCGEM1. Meanwhile， SW1990 transfected with siRNA to decrease the expression level of PCGEM1. Then， the abilities of the proliferation and migration were determined using clone formation assay， CCK-8 assay and cell migration assay， respectively. Furthermore， the protein levels of MMP2， MMP9 and Ecadherin were examined by Western blot and the expression levels of MMP2 and MMP9 were detected by qRTPCR.The results showed there were significant differences of PCGEM1 expression level in three kinds of human pancreatic cancer cell lines. The expression level of PCGEM1 in SW1990 was relatively high， compared with which was in BxPC3 and PaTu8988. The level of PCGEM1 in pancreatic cancer tissues was higher than that in adjacent non-cancer tissues. Here， it was shown that knockdown of PCGEM1 suppressed proliferation in vitro.In contrast， the overexpression of PCGEM1 stimulated these processes. The expression level of PCGEM1 was positively correlated with the migration ability of human pancreatic cancer cells. In addition， after up regulation of PCGEM1， the expression of MMP2 and MMP9 increased while the expression of E-cadherin decreased. Conversely，the expression of MMP2 and MMP9 decreased and E-cadherin decreased after down regulation of PCGEM1.Taken together， lncRNA PCGEM1 promotes the proliferation and migration of human pancreatic cancer cells.
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