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Effects of MBD2 on Proliferation and Migration of Human Pancreatic Cancer Cells
Zhang Youli1, Wang Zhen1, He Junbo1, Wei Hong1, Ge Lu1, Li jie1, Gong Aihua2,3, Xu Min1*
1Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China;
2State Key Laboratory of Oncogenes and Related Gene,Zhenjiang 212000, China;
3School of Medicine, Jiangsu University, Zhenjiang 212013, China
2State Key Laboratory of Oncogenes and Related Gene,Zhenjiang 212000, China;
3School of Medicine, Jiangsu University, Zhenjiang 212013, China
Abstract: This paper aimed at investigating the effects of methyl-CpG-binding domain protein 2 (MBD2) expression on proliferation and migration of human pancreatic cancer cells. The specific MBD2-shRNA was transiently transfected into the pancreatic cancer cells PaTu8988 and SW1990， respectively. Then， CCK-8 assay， colony formation assay and Transwell assay were performed to detect the cell proliferation， colony formation and migration rates in PaTu8988 and SW1990 cells， respectively. It was found that the expression of MBD2 was efficiently inhibited at both mRNA and protein levels in sh-MBD2 cells compared with control group (P<0.05). CCK-8， colony formation and Transwell assay revealed that MBD2 depletion decreased cell proliferation， colony formation and migration rates in PaTu8988 and SW1990 cells (P<0.05). Taken together， our results suggested that MBD2 gene silencing could inhibit the abilities of proliferation and migration in pancreatic cancer cell lines PaTu8988 and SW1990， which might provide an experimental basis for further study in the mechanism of MBD2 in pancreatic cancer.
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