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Isolation and Identification of Fetal Skin Stem Cells from Arbas Cashmere Goat
Nimantana, Liang Hongyu, Cang Ming, Guo Xudong, Liu Dongjun*, Shorgan*
Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education,Inner Mongolia University, Huhhot 010070, China
Abstract: In this study， fetal skin stem cells from Arbas Cashmere goat were isolated and cultured in vitro， in order to providing experimental materials for further research on hair follicle growth. Highly purified skin stem cells were isolated using enzyme digestion and adhesion to type IV collagen. The biological characteristics of skin stem cells were identified by morphological observation， growth curve， markers assay and differentiation in vitro. The results showed that the cells were in small cell size with typical cobblestone-like morphology， good adhesion capacity and high refractivity， and no significant morphological changes were appeared after 20 passages. Immunocytochemistry results showed the cells were positively expressed Krt15， CD34 and Itgβ1. The relative mRNA levels of Krt15， CD34 and Itgβ1 were 5.56， 24.37 and 7.22 times of their levels in Cashmere goat keratinocytes， respectively (P<0.01). After osteogenic and cartilage induction， the cells were positively stained by Von Kossa and alcian blue， respectively. The cells positively expressed MyoG after myogenic differentiation and cell fusion was observed by Hoechst 33342 staining. These results suggested that Arbas Cashmere goat fetal skin stem cell line was successfully established.
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