Home > Browse Issues > Vol.38 No.1
Latest Progress of Gene Knock-in Mediated by Programmable Nucleases in Mammals
Zhang Minjie1, Sun Ling1, Liu Zhen2, Cai Yijun2, Sun Qiang2, Yang Yufeng1, Chen Wenfeng1*
1Institute of Life Sciences, Fuzhou University, Fuzhou 350108, China;
2Institute of Neuroscience,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
2Institute of Neuroscience,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Abstract: Mammalian model animals have contributed significantly to the exploration of basic biology and human diseases. The feasibility to achieve genome engineering in mammals， especially targeted gene knockin (KI) is critical in this course. Zinc-finger nucleases (ZFN)， transcription activator-like effector nucleases (TALEN) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9-based RNA-guided DNA endonucleases (CRISPR/Cas9) are sparking a new revolution in biological research. These programmable nucleases enable relatively efficient and precise KI by inducing targeted DNA double-strand breaks (DSBs) that stimulate homologous recombination (HR) at the presence of homologous repair templates. Here， we review the principles of these technologies and their applications for gene KI in mammals. We also discuss latest strategies to elevate KI efficiency and reduce off-target ratio， which will enhance the overall performance of future transgenic practice.
CN
EN