Cultivation of Rat Brain Microvascular Endothelial Cells and the Expression of Drug Transporters in Primary Rat Brain Microvascular Endothelial Cells

Abstract: Rat brain microvascular endothelial cells (BMECs) were cultured with brain segment sticking mass methods. The BMECs were identified by observing under phase-contrast microscope， and by using immunocytochemical methods with anti-rat factor VIII antibody and CD34.The expression of organic anion transporting polypeptide2 (Oatp2) and P-glycoprotein (P-gp) were detected by immunocytochemical methods and Western blot analysis. In this study， the BMECs showed regular cobblestone and polygonal morphology and positive for VIIIF-Ag and CD34. The purity of positive stained cells was 90%. Both Oatp2 and P-gp expressed in the primary BMECs， and preminantly along the plasma membrane of endothelial cells. It is suggested that the rat BMECs were successfully cultured in vitro by brain segment sticking mass methods. The primary BMECs expressed the Oatp2 and P-gp. This experiment provide a possible pathway for studying transporters of the blood-brain barrier.
    

CSTR: 32200.14.cjcb.2007.04.0021