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The Effect of Compound Liguoric Tablets Water Extract on Maturation of Lipopolysaccharide-Induced Dendritic Cells
Luo Jiaojiao, Adila Aipire, Li Jinyu, Yuan Pengfei, Zhang Fuchun, Li Jinyao*
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology,Xinjiang University, Urumqi 830046, China
Abstract: Dendritic cell (DC) is professional antigen-presenting cell and links the innate and adaptive immune system. The strength and subtype of adaptive immune responses are dependent on the activated status of DC. Compound liguoric tablets (CLT) have been used to alleviate cough and phlegm. However， the effect of CLT on the maturation of DC need to be investigated. Here， we explored the effect of CLT water extract (CLTE) on the maturation and cytokine production of bone marrow-derived DC in the presence or absence of lipopolysaccharide (LPS). The levels of surface markers and the secretion of cytokines were detected by flow cytometry and ELISA，respectively. We found that 0.4 mg/mL CLTE decreased the proportion of gated cells， but increased the proportion of DCs in the gated cells. CLTE below 0.4 mg/mL concentrations inhibited the maturation of DC in a dosedependent manner， especially LPS-induced DC maturation. CLTE did not change the secretion of cytokines in the absence of LPS， but significantly reduced the production of IL-12p40 and IL-6 induced by LPS. These results suggested that CLT might exert the effect on the cough and phlegm via regulation the maturation of DC to reduce the airway inflammatory responses.
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