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hUC-MSCs Promote the Proliferation and Migration of Osteosarcoma Cells through IL-6/STAT3 Signaling
Hu Wenlong1,2,3, Yin Changchang1*, Wu Pingping2, Wu Yahua1, Wang Lili1, Ke Xiumei1
1Jiujiang Key Laboratory of Translational Medicine, Basic Medical College, Jiujiang University, Jiujiang 332000, China;
2Medicine Graduate School, Nanchang University, Nanchang 330006, China;
3Department of Orthopedics, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
2Medicine Graduate School, Nanchang University, Nanchang 330006, China;
3Department of Orthopedics, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
Abstract: This work was aimed to study the effects of IL-6/STAT3 signaling on the proliferation and migration of osteosarcoma cells. The expressions of interleukin-6 (IL-6) in human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and Saos-2 cells were assayed by ELISA. The protein levels of STAT3 and p-STAT3 in extracts from Saos-2 cells were determined by Western blot. RT-PCR was used to determine the transcription levels of PCNA， CyclinD1， Survivin and STAT3 genes. The proliferation of Saos-2 cells was measured by cytometry and CCK-8 assay， and migration potential by wound-healing assay and Transwell assay. The results demonstrated that the expression of IL-6 in hUC-MSCs was obviously higher than that in Saos-2 cells. STAT3 was activated by conditioned medium from hUC-MSCs (hUC-MSCs-CM) and IL-6 and the activation could be attenuated by a neutralization antibody against IL-6. The inhibition of STAT3 in Saos-2 cells by IL-6 neutralizing antibody or AG490 down-regulated the levels of mRNAs of PCNA， CyclinD1 and Survivin proliferation related genes related to proliferation， decreased cell proliferation and migration. The results proved that STAT3 activation by IL-6 from hUC-MSCs might promote the proliferation and migration of osteosarcoma cells in vitro.
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