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Effects of ADAM17 on Proliferation and Migration of Human Glioma Cells through Hippo Signaling Pathway
Zhang Chunli1, Han Xiu1, Xiong Ermeng1, Peng Wanxin1, Du Fengyi1, Zhou Hailang1, Gong Aihua1,2*
1Department of Basic Medical Sciences, School of Medicine, Jiangsu University, Zhenjiang 212013, China;
2State Key Laboratory for Oncogenes and Related Genes, Shanghai 200032, China
2State Key Laboratory for Oncogenes and Related Genes, Shanghai 200032, China
Abstract: To investigate the effects of ADAM17 on the ability of proliferation and migration of human glioma cells and its regulatory mechanism， we first detected ADAM17 expression in different glioma cell lines.Then the specific shRNA against ADAM17 and the expressing vector of pRK5M-ADAM17 were transfected into the glioma cells U87MG， U251MG and SW1783， respectively. q-PCR and Western blot were used to examine the levels of ADAM17 and the proteins related to Hippo signaling pathway. The relative rates of proliferation and migration were determined by CCK-8 assay and wound scratch assay， respectively. The results showed that ADAM17 depletion decreased the ability of proliferation and migration in U87MG， U251MG cells (P<0.05) and increased the levels of proteins related to Hippo signaling pathway. While ADAM17 over expression increased the ability of proliferation and migration in SW1783 cells (P<0.05). Taken together， our results suggested that the ADAM17 played an important role in the proliferation and migration of glioma cells. ADAM17 promoted the ability of proliferation and migration in glioma cells by inhibiting Hippo signaling pathway， reflecting in the decrease of MST2， p-MOB1， and the YAP phosphorylation.
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