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The Nuclear Export Sequence Cdc25B in Mouse
Hong-Mei Zhao1,2, Cheng Cui3, Xiao-Yan Xu2, Bing-Zhi Yu1*
1Department of Biochemistry, China Medical University, Shenyang 110001, China; 2Medical Laboratory, Liaoning Province People's Hospital, Shenyang 110016, China; 3Department of Physiology, China Medical University, Shenyang
Abstract: Aim to study the function of nuclear export sequence of cell division cycle 25 homolog B (Cdc25B) during mouse oocytes G2/M transition. mRNA of Cdc25B-WT， Cdc25B-Δ51， Cdc25B-Δ65 and fused plasmids of pEGFP-Cdc25B-WT， pEGFP-Cdc25B-Δ51， pEGFP-Cdc25B-Δ65 were microinjected into germinal vesicle stage mouse oocytes， the occurrence of germinal vesicle break down and subcellular localization of ectopic Cdc25B were observed. Results demonstrated that Cdc25B-Δ51 and Cdc25B-Δ65 all abrogated the function as meiosis inducer; Cdc25B-WT distributed mainly in cytoplasm while Cdc25B-Δ51 distributed in nuclear and cytoplasm， Cdc25B-Δ65 distributed in nuclear. Results suggested that a functional nuclear export sequence were amino acids between 52-65 in the N terminal region of Cdc25B.
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