The Effects of S100A9 on Proliferation and Migration of Human Squamous Cervical Carcinoma Hela Cell Line and Its Mechanisms

Abstract: To investigate the role of S100A9 in human squamous cervical carcinoma， the cervical carcinomam cells (Hela cell line) were infected with recombinant adenovirus expressing S100A9. Then， MTT was used to detect cell proliferation; Wound-healing test and Transwell assay were used to determine cell migration; Inverted microscope was used to observe the morphologic changes; Reverse transcription-PCR (RT-PCR) and Western blot were utilized to detect the mRNA or protein levels of E-Cadherin， Vimentin and the relevant molecules of Wnt/ β-catenin signaling pathway， respectively. The results showed that S100A9 over-expression promoted the proliferation and migration of Hela cells， and induced a morphology transition from a round cobblestone-like phenotype to a slender and fusiform appearance， also led to reduction of E-cadherin (P<0.05) and increase of Vimentin (P<0.01). Moreover， the levels of β-catenin， c-myc， Snail and Twist in S100A9 over-expressed Hela cells were also increased. The results demonstrated that S100A9 could promote the proliferation， migration and epithelial-mesenchymal transition (EMT) of Hela cells and also activated the Wnt/β-catenin signaling pathway. The mechanisms that S100A9 increased the cell proliferation and migration of Hela cell line might be associated with its promoting effect on EMT and the Wnt/β-catenin signaling pathway.

CSTR: 32200.14.cjcb.2015.12.0005