Home > Browse Issues > Vol.37 No.12
Comparative Study on Genetic Traits of Human Adipose， Umbilical Cord and Placenta Derived Mesenchymal Stem Cells during Subculture In Vitro
Liu Yu1, Ma Ming1, Hou Jun2, Gao Xuehua2, Chen Sixiang1, Liu Yue1, Deng Yin1, Zheng Dongming1, Tian Yi1, Zhang Rong3*, Chen Jingxian2*
1Chengdu Zhongchuang Qingke Medical Diagnosis Test Center, Chengdu 610094,China; 2Chengdu Qingke Biotechnology Co, Ltd, Chengdu 610094, China; 3Department of Biochemistry and Molecular Biology,
College of Basic Medicine and Forensic, Sichuan University, Chengdu 610041, China
Abstract: We isolated and cultured human umbilical cord derived mesenchymal stem cells (UC-MSCs)， adipose derived mesenchymal stem cells (AD-MSCs)， placenta derived mesenchymal stem cells (PMSCs) in vitro using the serum free culture medium. By comparison of the changes and differences in the biological characteristics and genetic characteristics among the three sources of mesenchymal stem cells (MSCs)， we can provide
experimental evidence and theoretical basis for clinical application of MSCs. By cell morphology observation， cell cycle detection， surface marker detection， chromosome karyotype analysis， gene expression and cytokine analysis， it was found that AD-MSCs and UC-MSCs were long spindle shaped adherent growth when cultured in vitro， while PMSCs was short spindle shaped or reticular cell adherent growth， and the nucleus was larger. Based on the longterm subculture and cell cycle analysis， we found that the ability of proliferation and differentiation of PMSCs were strongest， and UC-MSCs were stronger than AD-MSCs. The expressions of CD105， CD90 and CD73 were positive in all three kinds of cells， and the positive rate was 98%. The expressions of CD11b， CD19， CD34 and CD45 were lower than 1%， and the HLA-DR were all negative. The chromosomes in three kinds of cells were stable， and there were no signs of deletion， translocation and inversion. The expression of the 7 genes was different， but within the 7th passage， the expression of the gene was only slightly changed， and the cytokines in the passage were relatively stable. Our results demonstrated that within the 7th passage， PMSCs are safer than AD-MSCs and UC-MSCs while cultured in vitro with serum-free medium.
experimental evidence and theoretical basis for clinical application of MSCs. By cell morphology observation， cell cycle detection， surface marker detection， chromosome karyotype analysis， gene expression and cytokine analysis， it was found that AD-MSCs and UC-MSCs were long spindle shaped adherent growth when cultured in vitro， while PMSCs was short spindle shaped or reticular cell adherent growth， and the nucleus was larger. Based on the longterm subculture and cell cycle analysis， we found that the ability of proliferation and differentiation of PMSCs were strongest， and UC-MSCs were stronger than AD-MSCs. The expressions of CD105， CD90 and CD73 were positive in all three kinds of cells， and the positive rate was 98%. The expressions of CD11b， CD19， CD34 and CD45 were lower than 1%， and the HLA-DR were all negative. The chromosomes in three kinds of cells were stable， and there were no signs of deletion， translocation and inversion. The expression of the 7 genes was different， but within the 7th passage， the expression of the gene was only slightly changed， and the cytokines in the passage were relatively stable. Our results demonstrated that within the 7th passage， PMSCs are safer than AD-MSCs and UC-MSCs while cultured in vitro with serum-free medium.
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