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The Role of Plant DNA Break Repair Genes in Agrobacterium T-DNA Integration
Cui Hairui1*, Zhu Bin1, Song Yue1, Zhao Haijun1, Wang Qing2
1Institute of Nuclear-Agricultural Sciences, Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China;
2Wuxi Qiushi Bio-Agricultural Co.,Ltd., Wuxi 214105, China
2Wuxi Qiushi Bio-Agricultural Co.,Ltd., Wuxi 214105, China
Abstract: Transgenic plants have already played a great role in the development of crop new variety and biopharming. Agrobacterium-mediated genetic transformation has been widely used as a powerful tool for genome analysis and the predominant technology for production of transgenic plants. The gene transfer mediated by Agrobacterium is a very complex biological process involving the synergetic effect of genetic factors of both the bacterium and the host plant cell. Over the past two decades， a great deal has been learned about the molecular mechanism by which Agrobacterium produces T-DNA and transports it into the host nucleus， as well as the Agrobacterium-host plant interactions. However， T-DNA integration， the most critical step of the transformation process， largely remains an enigma. More and more evidences suggest that the DNA break repair genes of the host cell plays an important role in Agrobacterium T-DNA integration. In this review， the general process of Agrobacterium T-DNA transfer was introduced firstly and then we discussed the role of plant DNA break repair genes in T-DNA integration， to provide some useful information for the improvement of Agrobacterium-mediated transformation efficiency in plant by the genetic manipulation of DNA damage repair genes.
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