Home > Browse Issues > Vol.37 No.11
Automatic Detecting and Sorting White Blood Cells from the Whole Blood Sample via a Microfluidic Chip Device
Song Zhenyu1, Zhang Xiaojuan2, Song Yongxin3*
1Department of Radiotherapy, Jiaozhou Central Hospital of Qingdao, Qiangdao 266300, China;
2The Third People’s Hospital of Jiaozhou, Qiangdao 266300, China; 3Dalian Maritime University, Dalian 116026, China
2The Third People’s Hospital of Jiaozhou, Qiangdao 266300, China; 3Dalian Maritime University, Dalian 116026, China
Abstract: There is increasing demand on the white blood cells sorted from the whole blood sample in the biological and medical researches. We reports a microfluidic lab on chip device by which the white blood cells can be automatically and continually detected and sorted from the whole blood cells. While each cell of the sample solution passes through the resistive pulse sensing gate， a resistive signal pulse is generated. The magnitude of the signal represents the size of the cell. For the white blood cell which is larger in volume， a signal with larger magnitude is generated. The Labview program acquires the RPS signals and triggers the cell sorting process once a white blood cell is detected， thus sorting the white blood cell into the target channel. For the red blood cells， they are transported to the waste reservoir. The device described here is simple， automatic and label-free. It has the potential to become a portable device for white blood cell sorting.
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