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Effects of the Conditioned Medium after Culturing Endothelial Cell with High Concentration of Glucose on the Proliferation and Apoptosis of Human Umbilical Cord-derived Mesenchymal Stem Cell
Li Xun, Long Jiaoyue, Zhu Ming, Xing Wei, Guo Wei, Huang Hong, Xu Xiang*
State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Surgery Research, Daping Hospital,Third Military Medical University, Chongqing 400042, China
Abstract: MSC were cultured in the hUVEC-CM for 3 days， then their viability and proliferation were determined by RTCA
xCELLigence system， and apoptosis of the hUC-MSC was quantified by flow cytometry (Annexin V-FITC/PI
dual fluorescence). The protein levels of Bcl-2， Bax and Cl-Caspase3 were detected by Western blot. The results
demonstrated that compared with the control group， hUVEC-CM(HG) reduced viability and proliferation and
increased apoptosis of the hUC-MSC， which was associated with increased levels of Bax and Cl-Caspase3， and
decreased levels of Bcl-2. The results proved that hUVEC-CM(HG) induced apoptosis and inhibited the viability
and proliferation of hUC-MSC through regulating the expression of Bcl-2， Bax and Cl-Caspase3.
xCELLigence system， and apoptosis of the hUC-MSC was quantified by flow cytometry (Annexin V-FITC/PI
dual fluorescence). The protein levels of Bcl-2， Bax and Cl-Caspase3 were detected by Western blot. The results
demonstrated that compared with the control group， hUVEC-CM(HG) reduced viability and proliferation and
increased apoptosis of the hUC-MSC， which was associated with increased levels of Bax and Cl-Caspase3， and
decreased levels of Bcl-2. The results proved that hUVEC-CM(HG) induced apoptosis and inhibited the viability
and proliferation of hUC-MSC through regulating the expression of Bcl-2， Bax and Cl-Caspase3.
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