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Functional Dissection of MicroRNA in the Drosophila PGCs Fate Regulation
Liu Dongcheng, Qu Jie, Yang Huiming, Li Mengjie, Xuan Tao*, Li Mingfa
College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract: The Drosophila primordial germ cells (PGCs) are the germline stem cell precursors. The PGCs in larval gonads undergo a unique developmental process， which involves a regulatory pattern of cell fate and behavior. To systematically unravel the roles of miRNAs in PGCs fate regulation， we performed a miRNA expression profiling on developing larval gonads in female flies. The analysis identified a group of miRNAs expressing in the tissues. We next examined if those miRNA candidates function in the developmental process via the Gal4/UAS binary system. The results showed that miR-33 and miR-278 controled the PGCs differentiation in the larvae. The study in this paper provided a good working system for exploring functional miRNAs in the developmental contexts.
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