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Effect of Di(2-Ethylhexyl) Phthalate on the Functions of Human Spermatozoa In Vitro
Chen Wen1, Luo Tao2*
1Institute for Advanced Study, Nanchang University, Nanchang 330031, China; 2Institute of Life Science, Nanchang University, Nanchang 330031, China
Abstract: The aim of this study was to explore the effect of di(2-ethylhexyl) phthalate (DEHP) on the functions of human spermatozoa in vitro. Human ejaculated spermatozoa in vivo were exposed to DEHP at different concentrations (0， 0.1， 1， 10， 100 μmol/L). The viability， motility， hyperactivation and acrosome reaction (AR) of DEHP-treated spermatozoa were assessed by eosin-nigrosin staining， computer-aided sperm analysis， penetration of the artificial viscous medium and chlortetracycline staining. The results indicated that DEHP did not affect the viability of human spermatozoa， but it significantly inhibited the motility， hyperactivation and progesterone induced capacitation and AR of human spermatozoa. These results implied that in vitro exposure to DEHP inhibits the function of human spermatozoa in a short time.
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