Distribution of Tachyzoite and Histological Lesions in Kunming Mice Infected CT1 Strain of Toxoplasma gondii

Abstract: To study the distribution of tachyzoite and histological lesions in Kunming mice infected CT1 strains of Toxoplasma gondii， 1 oocyst/mice， 10 oocysts/mice and 100 oocysts/mice of CT1 Toxoplasma gondii were fed to Kunming mice. Mice were killed at diferent times after inoculation. Tissues were sampled and stained by H&E and immunohistochemistry (IHC) to observe the distribution of tachyzoite in Kunming mice. This study found that Kunming mice were susceptible to CT1 strain Toxoplasma gondii. The positive rate were 13.51%， 24.32% and 70.27% with increasing oocyst dose. Lethal dose 100 (LD100) is 100 oosysts and mice were died during 9~10 days after inoculation (DAI); within 0.5 h after inoculation (HAI)， sporozoites had excysted and penetrated some tissue of Kunming mice， the highest rate of tachyzoites positive tissue was mesenteric lymph nodes. There was widespread perivascular inflammation in acute infection period， and numerous cysts were found in the mice brain in chronic infection period. By observing the pathogenicity and dynamic distribution in organs of Kunming mice infected with CT-1 Toxoplasma gondii， this study provided basis for the research about Toxoplasma gondiiepidemiology and pathogenic mechanism in the further.

CSTR: 32200.14.cjcb.2015.10.0005