Protective Effect of HIF-1α Modified Bone Marrow Mesenchymal Stem Cells on Hypoxia-induced PC12 Cell Injury

Abstract: This article investigated the effect of HIF-1α-modified bone marrow mesenchymal stem cells (MSCs) on PC12 cells under hypoxia and its mechanism. PC12 cells were co-cultured with MSCs or HIF-1α- MSCs under hypoxic conditions， which were randomized into control group， hypoxia group， MSCs group， HIF-1α- MSCs group. Cell viability was measured at different time point by MTT assay. Morphological changes of PC12 cells were detected by HE staining; the apoptotic rates were detected by Hoechst 33258 staining and Annexin V-FITC assay; either mRNA or protein level of caspase-3 was detected by RT-PCR or immunofluorescence. The results of MTT showed that PC12 cell viability decreased with increasing hypoxia time; the cell viability decreased to 45.1% after hypoxia 12 h. Compared with the control group， the apoptosis rate， the RNA and protein levels of caspase-3 were increased in hypoxia group (P<0.05). Compared with the hypoxia group， MSCs and HIF-1α- MSCs could reduce apoptosis rate of PC12 cells and caspase-3 expression (P<0.05)， which were more effective in HIF-1α-MSCs group (P<0.05). It is proved that the HIF-1α-modified bone marrow mesenchymal stem cells enhanced protective effect of MSCs on PC12 cells damaged by hypoxia， which was related to the downregulation of caspase-3 expression.

CSTR: 32200.14.cjcb.2015.10.0003