Home > Browse Issues > Vol.37 No.9
Recent Advancement in the Generation of Genetically Modified Mammalian Models via CRISPR-Cas9
He Di1, Zhu Xianmin1,2, Xu Shuyang1, Xue Zhigang2, Fan Guoping3*
1School of Life Sciences and Technology, Tongji University, Shanghai 200092, China;
2Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China;
3Department of Human Genetics, University of California Los Angeles, CA 90095, USA
2Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China;
3Department of Human Genetics, University of California Los Angeles, CA 90095, USA
Abstract: Genetically modified animal models are important tools for understanding the gene function in development and disease. They have been widely used for modeling human pathophysiology， drug screening，efficacy evaluation and novel therapy development. However， the conventional technologies via homologous recombination in embryonic stem cell lines are time-consuming and labor-intense to generate genetically modified mammalian animal models. Recently， the emergence of CRISPR-Cas9 genome editing technology provides a much quick， precise， and site-specific method in the modification of mammalian genomes. This review will focus on the applications of CRISPR-Cas9 in the generation of a variety of genetically modified mammals.
CN
EN